The microscopes are part of the Electron Microscopy of Soft Matter Laboratory.
FEI Talos 200C
The FEI Talos 200C, our new cryo-TEM is field-emission gun (FEG)-equipped transmission electron microscope with a demonstrated resolution of 0.12 nm. It allows us to to perform tomography in the conventional TEM and the scanning-transmission electron microscopy (STEM) modes. This state-of-the-art TEM is also equipped with novel “phase-plates”, which convert phase differences between areas of the specimen to amplitude differences, thus enhancing image contrast without resolution loss in specimens of inherent little contrast. A Falcon III direct-imaging camera allows imaging at very low electron exposure, essential for electron-sensitive specimens, namely, most of our specimens.
FEI Tecnai T12 G2 Transmission Electron Microscope
This 120 kV, LaB6 emitter equipped, FEI T12 G2 cryo-dedicated transmission electron microscope (installed 2004), is equipped with two Gatan cooled-CCD cameras: a wide-angle, side-mounted, 1024×1024 pixel MultiScan 791 camera, and a high-resolution, bottom-mounted, 2048×2048 pixel UltraScan 1000 camera. We use either a Gatan 626 or Oxford CT3500 cryo-holders and transfer-station systems.
Zeiss Ultra plus HR-SEM
This high-resolution scanning electron microscope (HR-SEM) was installed in the Chemical Engineering Sobell Building in February 2008. Its Schotkky field emission electron gun provides excellent brightness, even at very low electron acceleration voltages, down to a few tens of volts. This is an important feature for high-resolution imaging of surface nanostructures, and for overcoming charging of uncoated non-conductive specimens. A Charge Compensation (CC) system improves that feature even further. The microscope is equipped with four imaging backscattered and secondary electron detectors: two of the in-lens type, and two outside the lens. It is also equipped with a Bruker Xflash x-ray energy dispersive spectrometer (EDS) for x-ray elemental microanalysis. A Bal-Tec cryo-stage allows imaging of cryogenic specimens, such as fast-cooled liquids and biological systems. All this makes a versatile and flexible tool for the complete nano- and microstructural analysis of a wide range of systems.
Olympus BH2 light microscope, connected to a Nikon DS-F12 CCD camera
The microscope is equipped for differential interference contrast (Nomarski) optics, phase-contrast, and polarized-light microscopy.