Microscopes

FEI Talos 200C

FEI Talos 200C

The FEI Talos 200C, our new cryo-TEM is field-emission gun (FEG)-equipped transmission electron microscope with a demonstrated resolution of 0.12 nm. It allows us to to perform tomography in the conventional TEM and the scanning-transmission electron microscopy (STEM) modes. This state-of-the-art TEM is also equipped with novel “phase-plates”, which convert phase differences between areas of the specimen to amplitude differences, thus enhancing image contrast without resolution loss in specimens of inherent little contrast. A Falcon III direct-imaging camera allows imaging at very low electron exposure, essential for electron-sensitive specimens, namely, most of our specimens.

FEI Tecnai T12 G2 Transmission Electron Microscope

FEI Tecnai T12 G2 Transmission Electron Microscope

This 120 kV, LaB6 emitter equipped, FEI T12 G2 cryo-dedicated transmission electron microscope (installed 2004), is equipped with two Gatan cooled-CCD cameras: a wide-angle, side-mounted, 1024×1024 pixel MultiScan 791 camera, and a high-resolution, bottom-mounted, 2048×2048 pixel UltraScan 1000 camera. We use either a Gatan 626 or Oxford CT3500 cryo-holders and transfer-station systems.

 

Zeiss Ultra plus HR-SEM

Zeiss Ultra plus HR-SEM

This high-resolution scanning electron microscope (HR-SEM) was installed in the Chemical Engineering Sobell Building in February 2008. Its Schotkky field emission electron gun provides excellent brightness, even at very low electron acceleration voltages, down to a few tens of volts. This is an important feature for high-resolution imaging of surface nanostructures, and for overcoming charging of uncoated non-conductive specimens. A Charge Compensation (CC) system improves that feature even further. The microscope is equipped with four imaging backscattered and secondary electron detectors: two of the in-lens type, and two outside the lens. It is also equipped with a Bruker Xflash x-ray energy dispersive spectrometer (EDS) for x-ray elemental microanalysis. A Bal-Tec cryo-stage allows imaging of cryogenic specimens, such as fast-cooled liquids and biological systems. All this makes a versatile and flexible tool for the complete nano- and microstructural analysis of a wide range of systems.

 

Olympus BH2 light microscope, connected to a Nikon DS-F12 CCD camera

Olympus BH2 light microscope, connected to a Nikon DS-F12 CCD camera

The microscope is equipped for differential interference contrast (Nomarski) optics, phase-contrast, and polarized-light microscopy.